Pilot-plant scale extraction of phenolic compounds from grape canes: Comprehensive characterization by LC-ESI-LTQ-Orbitrap-MS

Grape canes, also named vine shoots, are well-known viticultural byproducts containing high levels of phenolic compounds, which associated with a broad range health benefits. In this work, grape canes (Vitis vinifera cv. Pinot noir) were extracted in 750 L pilot-plant reactor under the following conditions: temperature 80 °C, time 100 min, solid/liquid ratio 1:10. The comprehensive characterization cane compounds was performed by liquid chromatography coupled to high-resolution/accurate mass measurement LTQ-Orbitrap spectrometry. A total 44 identified and, 26 them quantified, consisting acids and aldehydes (17), flavonoids (12), stilbenoids (15). most abundant class phenolics stilbenoids, among (E)-?-viniferin predominated. profile obtained using pilot plant extraction differed significantly from results laboratory-scale studies previously. Additionally, we observed antioxidant capacity extract measured radical scavenging potential (ABTS+) (2209 ± 125 µmol TE/g DW) oxygen absorbance fluorescein (ORAC-FL) (4612 155 DW). for their may be used as by-product development novel nutraceutical pharmaceutical products, improving value sustainability these residues.